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MYO1C Polyclonal antibody

MYO1C Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17969-1-AP

Print datasheet

Synonyms

myr2, myosin IC, Myosin I beta, MMI-beta, MMIb

验证数据展示

  • IHC staining of human colon cancer using 17969-1-AP

    IHC staining of human colon cancer using 17969-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17969-1-AP (MYO1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO1C antibody included
  • IHC staining of human kidney using 17969-1-AP

    IHC staining of human kidney using 17969-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17969-1-AP (MYO1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO1C antibody included
  • IHC staining of mouse colon using 17969-1-AP

    IHC staining of mouse colon using 17969-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17969-1-AP (MYO1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO1C antibody included
  • IHC staining of mouse colon using 17969-1-AP

    IHC staining of mouse colon using 17969-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17969-1-AP (MYO1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO1C antibody included
  • IHC staining of rat colon using 17969-1-AP

    IHC staining of rat colon using 17969-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 17969-1-AP (MYO1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO1C antibody included
  • IHC staining of rat colon using 17969-1-AP

    IHC staining of rat colon using 17969-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 17969-1-AP (MYO1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO1C antibody included
  • IF Staining of U2OS using 17969-1-AP

    IF Staining of U2OS using 17969-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using MYO1C antibody (17969-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman kidney tissue, human colon cancer tissue, mouse colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy MYO1C Ready-To-Use IHC Kit. MYO1C primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17969-1-AP targets MYO1C in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MYO1C fusion protein Ag11970 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin IC
Synonyms myr2, myosin IC, Myosin I beta, MMI-beta, MMIb
Calculated Molecular Weight 1028 aa, 118 kDa
GenBank Accession NumberBC068013
Gene Symbol MYO1C
Gene ID (NCBI) 4641
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00159
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for MYO1C antibody 17969-1-APDownload protocol
IF protocol for MYO1C antibody 17969-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols