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MYO1E Monoclonal antibody

MYO1E Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A12F5

Cat No : 68152-1-Ig

Print datasheet

Synonyms

HuncM IC, MYO1E, Myosin Ic, myosin IE

验证数据展示

  • WB analysis using 68152-1-Ig

    WB analysis using 68152-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68152-1-Ig (MYO1E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells. HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy MYO1E Ready-To-Use IHC Kit. MYO1E primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68152-1-Ig targets MYO1E in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen MYO1E fusion protein Ag12244 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin IE
Synonyms HuncM IC, MYO1E, Myosin Ic, myosin IE
Calculated Molecular Weight 1108 aa, 127 kDa
Observed Molecular Weight120-150 kDa
GenBank Accession NumberBC098392
Gene Symbol MYO1E
Gene ID (NCBI) 4643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ12965
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYO1E, also named as Myosin-Ie and MYO1C (distinct from the MYO1C gene located on chromosome 17), encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in this gene are the cause of focal segmental glomerulosclerosis-6.

实验方案

Product Specific Protocols
WB protocol for MYO1E antibody 68152-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols