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MYO1F Polyclonal antibody

MYO1F Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28841-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 28841-1-AP

    WB analysis using 28841-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28841-1-AP (MYO1F antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells, rat spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28841-1-AP targets MYO1F in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MYO1F fusion protein Ag29899 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin IF
Synonyms
Calculated Molecular Weight 1098 aa, 125 kDa
Observed Molecular Weight 115-125 kDa
GenBank Accession NumberBC028071
Gene Symbol MYO1F
Gene ID (NCBI) 4542
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00160
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myosin family members are proteins that hydrolyze ATP to generate force or directional movement along actin filaments and are implicated in the rearrangement of the cytoskeleton in several cell types. Myosin 1F (MYO1F), a long tailed class I myosin, is highly expressed in natural killer (NK) cells, macrophages, dendritic cells, and neutrophils. MYO1F plays a critical role in the mediating of signaling molecules "trafficking from membrane to cytoplasm," and this process is essential for the antifungal signaling pathway activation.

实验方案

Product Specific Protocols
WB protocol for MYO1F antibody 28841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols