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验证数据展示

  • WB analysis using 25772-1-AP

    WB analysis using 25772-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25772-1-AP (MYO1G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 25772-1-AP

    IF Staining of Jurkat using 25772-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (25772-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

MYO1G Polyclonal antibody

MYO1G Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25772-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

HA 2, MYO1G, myosin IG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, Raji cells
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy MYO1G Ready-To-Use IHC Kit. MYO1G primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25772-1-AP targets MYO1G in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MYO1G fusion protein Ag22696 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin IG
别名 HA 2, MYO1G, myosin IG
计算分子量 1018 aa, 116 kDa
观测分子量110-120 kDa
GenBank蛋白编号BC113544
基因名称 MYO1G
Gene ID (NCBI) 64005
RRIDAB_3085822
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDB0I1T2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYO1G is a plasma membrane-associated class I myosin that is abundant in T and B lymphocytes and mast cells and expressed specifically in the haematopoietic system. Class I myosins have been implicated in various cellular processes relying on actin-dependent membrane dynamics such as endocytosis, secretion, adhesion, motility and regulation of mechanosensitive channels (PMID: 19968988). MYO1G localizes to the plasma membrane linked to PIP2 and PIP3, is particularly enriched at cell-surface microvilli, and associates in an ATP-releasable manner to the actin cytoskeleton (PMID: 24310084). MYO1G can be detected as about 110-120 kDa.

实验方案

Product Specific Protocols
WB protocol for MYO1G antibody 25772-1-APDownload protocol
IF protocol for MYO1G antibody 25772-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols