• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MYO1G Recombinant antibody, PBS Only

MYO1G Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240503E1

Cat no : 83556-5-PBS

Print datasheet

Synonyms

Unconventional myosin-Ig, myosin IG, Minor histocompatibility antigen HA-2, HA 2, 240503E1

验证数据展示

  • WB analysis using 83556-5-RR (same clone as 83556-5-PBS)

    WB analysis using 83556-5-RR (same clone as 83556-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83556-5-RR (MYO1G antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    IF Staining of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (83556-5-RR, Clone: 240503E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    IF Staining of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (83556-5-RR, Clone: 240503E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    FC experiment of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MYO1G Recombinant antibody (83556-5-RR, Clone:240503E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83556-5-RR

    Affinity and Kinetic Characterization of 83556-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83556-5-RR against Human MYO1G were performed. The affinity constant is 1.03 nM.

Planning an IHC experiment? We recommend our IHCeasy MYO1G Ready-To-Use IHC Kit. MYO1G primary antibody included.

产品信息

83556-5-PBS targets MYO1G in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen MYO1G fusion protein Ag22698 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myosin IG
Synonyms Unconventional myosin-Ig, myosin IG, Minor histocompatibility antigen HA-2, HA 2, 240503E1
Calculated Molecular Weight 1018 aa, 116 kDa
Observed Molecular Weight110-120 kDa
GenBank Accession NumberBC113544
Gene Symbol MYO1G
Gene ID (NCBI) 64005
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDB0I1T2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYO1G is a plasma membrane-associated class I myosin that is abundant in T and B lymphocytes and mast cells and expressed specifically in the haematopoietic system. Class I myosins have been implicated in various cellular processes relying on actin-dependent membrane dynamics such as endocytosis, secretion, adhesion, motility and regulation of mechanosensitive channels (PMID: 19968988). MYO1G localizes to the plasma membrane linked to PIP2 and PIP3, is particularly enriched at cell-surface microvilli, and associates in an ATP-releasable manner to the actin cytoskeleton (PMID: 24310084). MYO1G can be detected as about 110-120 kDa.