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Host
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 83556-5-RR (same clone as 83556-5-PBS)

    WB analysis using 83556-5-RR (same clone as 83556-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83556-5-RR (MYO1G antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    IF Staining of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (83556-5-RR, Clone: 240503E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    IF Staining of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MYO1G antibody (83556-5-RR, Clone: 240503E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    FC experiment of Jurkat using 83556-5-RR (same clone as 83556-5-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug MYO1G Recombinant antibody (83556-5-RR, Clone:240503E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83556-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83556-5-RR

    Affinity and Kinetic Characterization of 83556-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83556-5-RR against Human MYO1G were performed. The affinity constant is 1.03 nM.

MYO1G Recombinant antibody, PBS Only

MYO1G Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83556-5-PBS

产品说明书

CloneNo. 240503E1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Unconventional myosin-Ig, myosin IG, Minor histocompatibility antigen HA-2, HA 2, 240503E1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MYO1G Ready-To-Use IHC Kit. MYO1G primary antibody included.

产品信息

83556-5-PBS targets MYO1G in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 MYO1G fusion protein Ag22698 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myosin IG
别名 Unconventional myosin-Ig, myosin IG, Minor histocompatibility antigen HA-2, HA 2, 240503E1
计算分子量 1018 aa, 116 kDa
观测分子量110-120 kDa
GenBank蛋白编号BC113544
基因名称 MYO1G
Gene ID (NCBI) 64005
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDB0I1T2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYO1G is a plasma membrane-associated class I myosin that is abundant in T and B lymphocytes and mast cells and expressed specifically in the haematopoietic system. Class I myosins have been implicated in various cellular processes relying on actin-dependent membrane dynamics such as endocytosis, secretion, adhesion, motility and regulation of mechanosensitive channels (PMID: 19968988). MYO1G localizes to the plasma membrane linked to PIP2 and PIP3, is particularly enriched at cell-surface microvilli, and associates in an ATP-releasable manner to the actin cytoskeleton (PMID: 24310084). MYO1G can be detected as about 110-120 kDa.