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MYO5A Polyclonal antibody

MYO5A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55318-1-AP

Print datasheet

Synonyms

GS1, MYH12, MYO5A, Myosin 12, Myosin heavy chain 12, Myosin Va, Myoxin, MYR12

验证数据展示

  • WB analysis using 55318-1-AP

    WB analysis using 55318-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55318-1-AP (MYO5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 55318-1-AP

    IHC staining of mouse brain using 55318-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55318-1-AP (MYO5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 55318-1-AP

    IHC staining of mouse brain using 55318-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55318-1-AP (MYO5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55318-1-AP targets MYO5A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin VA (heavy chain 12, myoxin)
Synonyms GS1, MYH12, MYO5A, Myosin 12, Myosin heavy chain 12, Myosin Va, Myoxin, MYR12
Calculated Molecular Weight 215 kDa
Observed Molecular Weight210-220 kDa
GenBank Accession NumberNM_000259
Gene Symbol MYO5A
Gene ID (NCBI) 4644
RRIDAB_2918356
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4I1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYO5A antibody 55318-1-APDownload protocol
IHC protocol for MYO5A antibody 55318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols