验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human colon tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29996-1-AP targets MYO5B in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | MYO5B fusion protein Ag31590 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | myosin VB |
Synonyms | KIAA1119, MYO5B, myosin VB |
Calculated Molecular Weight | 214KD |
Observed Molecular Weight | 200 kDa |
GenBank Accession Number | NM_001080467 |
Gene Symbol | MYO5B |
Gene ID (NCBI) | 4645 |
RRID | AB_2935496 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9ULV0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYO5B is a member of the class V of unconventional, dimeric nonfilamentous myosins, which may be involved in vesicular trafficking. Members of the Rab family of small GTPases, consisting of Rab11a, Rab11b, and Rab25, have recently emerged as potential mediators of vesicle transport by MYO5B (PMID: 21282656). MYO5B is required for polarization of hepatocytes and mutations of MYO5B could disrupt epithelial cell polarity. Loss-of-Function of MYO5B caused by mutation is the main cause of microvillus inclusion disease (MVID). Recently it was reported that the expression of MYO5B was downregulated in gastric cancer and inactivation of MYO5B may contribute to tumorigenesis (PMID: 22134786).
实验方案
Product Specific Protocols | |
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WB protocol for MYO5B antibody 29996-1-AP | Download protocol |
IHC protocol for MYO5B antibody 29996-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |