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MYO5B Polyclonal antibody

MYO5B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29996-1-AP

Print datasheet

Synonyms

KIAA1119, MYO5B, myosin VB

验证数据展示

  • WB analysis using 29996-1-AP

    WB analysis using 29996-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29996-1-AP (MYO5B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 29996-1-AP

    IHC staining of human colon using 29996-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO5B antibody included
  • IHC staining of human colon using 29996-1-AP

    IHC staining of human colon using 29996-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO5B antibody included
  • IHC staining of mouse colon using 29996-1-AP

    IHC staining of mouse colon using 29996-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29996-1-AP (MYO5B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYO5B antibody included

经过测试的应用

Positive WB detected inHepG2 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MYO5B Ready-To-Use IHC Kit. MYO5B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29996-1-AP targets MYO5B in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen MYO5B fusion protein Ag31590 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin VB
Synonyms KIAA1119, MYO5B, myosin VB
Calculated Molecular Weight214KD
Observed Molecular Weight 200 kDa
GenBank Accession NumberNM_001080467
Gene Symbol MYO5B
Gene ID (NCBI) 4645
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULV0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYO5B is a member of the class V of unconventional, dimeric nonfilamentous myosins, which may be involved in vesicular trafficking. Members of the Rab family of small GTPases, consisting of Rab11a, Rab11b, and Rab25, have recently emerged as potential mediators of vesicle transport by MYO5B (PMID: 21282656). MYO5B is required for polarization of hepatocytes and mutations of MYO5B could disrupt epithelial cell polarity. Loss-of-Function of MYO5B caused by mutation is the main cause of microvillus inclusion disease (MVID). Recently it was reported that the expression of MYO5B was downregulated in gastric cancer and inactivation of MYO5B may contribute to tumorigenesis (PMID: 22134786).

实验方案

Product Specific Protocols
WB protocol for MYO5B antibody 29996-1-APDownload protocol
IHC protocol for MYO5B antibody 29996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols