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MYO9B Recombinant antibody

MYO9B Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241163D8

Cat no : 84090-4-RR

Print datasheet

Synonyms

Unconventional myosin-9b, MYR5, myosin IXB, MYO9B variant protein, CELIAC4

验证数据展示

  • WB analysis using 84090-4-RR

    WB analysis using 84090-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84090-4-RR (MYO9B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84090-4-RR

    FC experiment of A431 using 84090-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MYO9B Recombinant antibody (84090-4-RR, Clone:241163D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells, HL-60 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy MYO9B Ready-To-Use IHC Kit. MYO9B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84090-4-RR targets MYO9B in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen MYO9B fusion protein Ag33349 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myosin IXB
Synonyms Unconventional myosin-9b, MYR5, myosin IXB, MYO9B variant protein, CELIAC4
Calculated Molecular Weight 2023 aa, 223 kDa
Observed Molecular Weight 230-250 kDa
GenBank Accession NumberBC018108
Gene Symbol MYO9B
Gene ID (NCBI) 4650
RRIDAB_3671656
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13459
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYO9B antibody 84090-4-RRDownload protocol
FC protocol for MYO9B antibody 84090-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols