验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, human stomach tissue, mouse skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
14238-1-AP targets Myocilin in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Myocilin fusion protein Ag5492 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myocilin, trabecular meshwork inducible glucocorticoid response |
| 别名 | GLC1A, GPOA, JOAG, JOAG1, MYOC, Myocilin, TIGR |
| 计算分子量 | 57 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC029261 |
| 基因名称 | Myocilin |
| Gene ID (NCBI) | 4653 |
| RRID | AB_2148652 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99972 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622). MYOC has a molecular weight of 55-57 kDa, besides, a 66-kDa form, which could be a result of post-translational modifications, has also been reported in some researches (PMID: 9497363; 12150989).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Myocilin antibody 14238-1-AP | Download protocol |
| IP protocol for Myocilin antibody 14238-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ann Transl Med Adenosine A3 receptor activated in H2O2 oxidative stress of primary open-angle glaucoma. | ||
Int J Ophthalmol Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork | ||
J Ocul Pharmacol Ther Activation of Sonic Hedgehog Signaling Pathway Regulates Human Trabecular Meshwork Cell Function | ||
Int J Mol Sci The Delayed Turnover of Proteasome Processing of Myocilin upon Dexamethasone Stimulation Introduces the Profiling of Trabecular Meshwork Cells' Ubiquitylome | ||
Discov Oncol Identification of KRT16 and ANXA10 as cell cycle regulation genes for lung adenocarcinoma based on self-transcriptome sequencing of surgical samples and TCGA public data mining | ||
