MYOD1 Monoclonal antibody

MYOD1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5D1D12

Cat no : 66214-1-Ig

Print datasheet

Synonyms

bHLHc1, Myf 3, MYF3, MYOD, MYOD1, myogenic differentiation 1, Myogenic factor 3, PUM



经过测试的应用

Positive WB detected inhuman skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 5 publications below

产品信息

66214-1-Ig targets MYOD1 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, pig
Immunogen MYOD1 fusion protein Ag13512 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myogenic differentiation 1
Synonyms bHLHc1, Myf 3, MYF3, MYOD, MYOD1, myogenic differentiation 1, Myogenic factor 3, PUM
Calculated Molecular Weight 320 aa, 35 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC064493
Gene Symbol MYOD1
Gene ID (NCBI) 4654
RRIDAB_2881605
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15172
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calculated molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45-47 kDa. (PMID: 12037670)

实验方案

Product Specific Protocols
WB protocol for MYOD1 antibody 66214-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

Vitellogenin 2 promotes muscle development and stimulates the browning of white fat

Authors - Yilei Li
humanWB

Front Oncol

Deoxypodophyllotoxin Inhibits Non-Small Cell Lung Cancer Cell Growth by Reducing HIF-1α-Mediated Glycolysis.

Authors - Yuping Yang
mouseWB

Toxicol Appl Pharmacol

A novel oleanolic acid derivative HA-19 ameliorates muscle atrophy via promoting protein synthesis and preventing protein degradation.

Authors - Wei Cui
ratWB

Int J Stem Cells

Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells

Authors - Jingcheng Wang
pigWB

Anim Biotechnol

Retinoic acid signalling inhibits myogenesis by blocking MYOD translation in pig skeletal muscle cells

Authors - Changying Wang