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MYOM1 Polyclonal antibody

MYOM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22084-1-AP

Print datasheet

Synonyms

MYOM1, Myomesin 1, myomesin 1, 185kDa, Myomesin family member 1, SKELEMIN

验证数据展示

  • WB analysis of mouse heart using 22084-1-AP

    WB analysis of mouse heart using 22084-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 22084-1-AP (MYOM1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 22084-1-AP

    IHC staining of mouse heart using 22084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 22084-1-AP

    IHC staining of mouse heart using 22084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22084-1-AP targets MYOM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MYOM1 fusion protein Ag17263 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myomesin 1, 185kDa
Synonyms MYOM1, Myomesin 1, myomesin 1, 185kDa, Myomesin family member 1, SKELEMIN
Calculated Molecular Weight 1685 aa, 188 kDa
Observed Molecular Weight 170-180 kDa
GenBank Accession NumberBC116183
Gene Symbol MYOM1
Gene ID (NCBI) 8736
RRIDAB_2878989
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52179
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYOM1 is a major component of the vertebrate myofibrillar M band. MYOM1 binds myosin, titin, and light meromyosin. This binding is dose-dependent.

实验方案

Product Specific Protocols
WB protocol for MYOM1 antibody 22084-1-APDownload protocol
IHC protocol for MYOM1 antibody 22084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols