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验证数据展示

  • WB analysis using 20360-1-AP

    WB analysis using 20360-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 20360-1-AP

    IHC staining of human skeletal muscle using 20360-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 20360-1-AP

    IHC staining of human skeletal muscle using 20360-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of mouse skeletal muscle using 20360-1-AP

    IF Staining of mouse skeletal muscle using 20360-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of C2C12 using 20360-1-AP

    IF Staining of C2C12 using 20360-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using MYOM1-Specific antibody (20360-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of mouse skeletal muscle using 20360-1-AP

    WB analysis of mouse skeletal muscle using 20360-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

MYOM1-Specific Polyclonal antibody

MYOM1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 20360-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (2)

别名

MYOM1, Myomesin-1, Myomesin family member 1, Myomesin 1, 190 kDa titin-associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue, mouse heart tissue
Positive IF/ICC detected inC2C12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20360-1-AP targets MYOM1-Specific in WB, IHC, IF/ICC, IF-P, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC, RIP, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myomesin 1, 185kDa
别名 MYOM1, Myomesin-1, Myomesin family member 1, Myomesin 1, 190 kDa titin-associated protein
计算分子量 188 kDa
观测分子量 190 kDa
GenBank蛋白编号NM_003803
基因名称 MYOM1
Gene ID (NCBI) 8736
RRIDAB_10696312
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52179
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYOM1 is a major component of the vertebrate myofibrillar M band. MYOM1 binds myosin, titin, and light meromyosin. This binding is dose-dependent. The antibody is specific to MYOM1.

实验方案

Product Specific Protocols
WB protocol for MYOM1-Specific antibody 20360-1-APDownload protocol
IHC protocol for MYOM1-Specific antibody 20360-1-APDownload protocol
IF protocol for MYOM1-Specific antibody 20360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Rep

Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.

Authors - Catarina Rippe
View Article
mouseWB

J Cell Mol Med

Hydrogen sulphide ameliorating skeletal muscle atrophy in db/db mice via Muscle RING finger 1 S-sulfhydration.

Authors - Fangping Lu
View Article
mouseWB,RIP

iScience

Splicing factor Srsf5 deletion disrupts alternative splicing and causes noncompaction of ventricular myocardium

Authors - Xiaoli Zhang, Ze
View Article
ratIHC,IF

Cell Signal

Long non-coding RNA Pvt1 modulates the pathological cardiac hypertrophy via miR-196b-mediated OSMR regulation.

Authors - Qingqing Wu
View Article
ratIF

Int J Mol Sci

Skeletal Muscle Dysfunction in Experimental Pulmonary Hypertension

Authors - Kosmas Kosmas
View Article