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MYOM3 Recombinant antibody

MYOM3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240981F9

Cat no : 83852-3-RR

Print datasheet

Synonyms

Myomesin-3, Myomesin family member 3, Myomesin 3, 240981F9

验证数据展示

  • WB analysis using 83852-3-RR

    WB analysis using 83852-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83852-3-RR (MYOM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse skeletal muscle using 83852-3-RR

    IF Staining of mouse skeletal muscle using 83852-3-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYOM3 antibody (83852-3-RR, Clone: 240981F9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse skeletal muscle using 83852-3-RR

    IHC staining of mouse skeletal muscle using 83852-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 83852-3-RR (MYOM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83852-3-RR

    Affinity and Kinetic Characterization of 83852-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83852-3-RR against Human MYOM3 were performed. The affinity constant is 0.106 nM.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83852-3-RR is MYOM3 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MYOM3 fusion protein Ag11849 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name myomesin family, member 3
Synonyms Myomesin-3, Myomesin family member 3, Myomesin 3, 240981F9
Calculated Molecular Weight 162 kDa, 136 kDa
Observed Molecular Weight 162 kDa
GenBank Accession NumberBC067101
Gene Symbol MYOM3
Gene ID (NCBI) 127294
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ5VTT5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYOM3 (myomesin 3) is a structural component of the M-band in striated muscle and is involved in sarcomere stability and resistance during intense or sustained stretching. MYOM3 can be detected mainly in intermediate-speed fibers of skeletal muscle. Recently high levels of MYOM3 fragments were detected in sera from patients with muscular dystrophy, including Duchenne muscular dystrophy (DMD). MYOM3 fragments may be used as serum biomarkers for DMD and other neuromuscular disorders.

实验方案

Product Specific Protocols
WB protocol for MYOM3 antibody 83852-3-RRDownload protocol
IHC protocol for MYOM3 antibody 83852-3-RRDownload protocol
IF protocol for MYOM3 antibody 83852-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols