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MYOZ1 Polyclonal antibody

MYOZ1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13160-1-AP

Print datasheet

Synonyms

Calsarcin 2, CS 2, FATZ, MYOZ, MYOZ1, myozenin 1, Protein FATZ

验证数据展示

  • WB analysis of mouse skeletal muscle using 13160-1-AP

    WB analysis of mouse skeletal muscle using 13160-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13160-1-AP (MYOZ1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IP experiment of mouse skeletal muscle using 13160-1-AP

    IP experiment of mouse skeletal muscle using 13160-1-AP

    IP result of anti-MYOZ1 (IP:13160-1-AP, 4ug; Detection:13160-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, human heart tissue
Positive IP detected inmouse skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

13160-1-AP targets MYOZ1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat, fish
Immunogen MYOZ1 fusion protein Ag3806 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myozenin 1
Synonyms Calsarcin 2, CS 2, FATZ, MYOZ, MYOZ1, myozenin 1, Protein FATZ
Calculated Molecular Weight 299 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC025753
Gene Symbol MYOZ1
Gene ID (NCBI) 58529
RRIDAB_2282208
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP98
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myozenin-1 is a protein that in humans is encoded by the MYOZ1 gene. MYOZ1 has been shown to interact with Telethonin, and Actinin, alpha. The MW of this protein is 32 kDa, and Catalog#13160-1-AP specially recognises the 32 kDa protein.

实验方案

Product Specific Protocols
WB protocol for MYOZ1 antibody 13160-1-APDownload protocol
IP protocol for MYOZ1 antibody 13160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Front Physiol

NO-Dependent Mechanisms of Myosin Heavy Chain Transcription Regulation in Rat Soleus Muscle After 7-Days Hindlimb Unloading.

Authors - Kristina A Sharlo
View Article
fishWB

J Muscle Res Cell Motil

Reduced expression of MyHC slow isoform in rat soleus during unloading is accompanied by alterations of endogenous inhibitors of calcineurin/NFAT signaling pathway.

Authors - Yulia N Lomonosova
View Article
ratWB

J Appl Physiol (1985)

Plantar mechanical stimulation prevents calcineurin/NFATc1 inactivation and slow-to-fast fiber-type shift in rat soleus muscle under hindlimb unloading.

Authors - Kristina Sharlo
View Article