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MYPN-Specific Polyclonal antibody

MYPN-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16180-1-AP

Print datasheet

Synonyms

145 kDa sarcomeric protein, MYOP, myopalladin, MYPN

验证数据展示

  • WB analysis of HeLa using 16180-1-AP

    WB analysis of HeLa using 16180-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16180-1-AP

    IF Staining of HepG2 using 16180-1-AP

    Immunofluorescent analysis of HepG2 cells using 16180-1-AP (MYPN-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human skeletal muscle using 16180-1-AP

    IHC staining of human skeletal muscle using 16180-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 16180-1-AP

    IHC staining of human skeletal muscle using 16180-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16180-1-AP targets MYPN-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myopalladin
Synonyms 145 kDa sarcomeric protein, MYOP, myopalladin, MYPN
Calculated Molecular Weight 145 kDa
Observed Molecular Weight 145 kDa
GenBank Accession NumberNM_032578
Gene Symbol MYPN
Gene ID (NCBI) 84665
RRIDAB_2146767
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86TC9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYPN is a component of the sarcomere that tethers nebulin in skeletal muscle and nebulette in cardiac muscle to alpha-actinin at the Z lines (from OMIM). Myopalladin and palladin share C-terminal fragment in sequence. While myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. This antibody is specific to myopalladin.

实验方案

Product Specific Protocols
WB protocol for MYPN-Specific antibody 16180-1-APDownload protocol
IHC protocol for MYPN-Specific antibody 16180-1-APDownload protocol
IF protocol for MYPN-Specific antibody 16180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.

Authors - Hexiao Shen
View Article