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验证数据展示

  • WB analysis of HeLa using 13751-1-AP

    WB analysis of HeLa using 13751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 13751-1-AP

    WB analysis of Jurkat using 13751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 13751-1-AP

    IP experiment of HeLa using 13751-1-AP

    IP result of anti-MYST2 (IP:13751-1-AP, 4ug; Detection:13751-1-AP 1:500) with HeLa cells lysate 2480ug.

  • IF Staining of NIH/3T3 using 13751-1-AP

    IF Staining of NIH/3T3 using 13751-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 13751-1-AP (MYST2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human pancreas cancer using 13751-1-AP

    IHC staining of human pancreas cancer using 13751-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAT7/MYST2 antibody included
  • IHC staining of human pancreas cancer using 13751-1-AP

    IHC staining of human pancreas cancer using 13751-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAT7/MYST2 antibody included
  • IHC staining of human thyroid cancer using 13751-1-AP

    IHC staining of human thyroid cancer using 13751-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAT7/MYST2 antibody included
  • IHC staining of human thyroid cancer using 13751-1-AP

    IHC staining of human thyroid cancer using 13751-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAT7/MYST2 antibody included
  • IHC staining of mouse stomach using 13751-1-AP

    IHC staining of mouse stomach using 13751-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAT7/MYST2 antibody included
  • IHC staining of mouse stomach using 13751-1-AP

    IHC staining of mouse stomach using 13751-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAT7/MYST2 antibody included
  • IHC staining of rat stomach using 13751-1-AP

    IHC staining of rat stomach using 13751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAT7/MYST2 antibody included
  • IHC staining of rat stomach using 13751-1-AP

    IHC staining of rat stomach using 13751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAT7/MYST2 antibody included
  • IHC staining of rat testis using 13751-1-AP

    IHC staining of rat testis using 13751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAT7/MYST2 antibody included
  • IHC staining of rat testis using 13751-1-AP

    IHC staining of rat testis using 13751-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAT7/MYST2 antibody included
  • IHC staining of human pancreas cancer using 13751-1-AP

    IHC staining of human pancreas cancer using 13751-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13751-1-AP (MYST2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KAT7/MYST2 antibody included
  • Featured Product
  • KD/KO验证

MYST2 Polyclonal antibody

MYST2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13751-1-AP

产品说明书

引用文献(18)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

别名

KAT7, KAT 7, Histone acetyltransferase KAT7, HBOa, HBO1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human testis tissue, Jurkat cells, NIH/3T3 cells, MCF-7 cells, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human thyroid cancer tissue, mouse stomach tissue, rat stomach tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy KAT7/MYST2 Ready-To-Use IHC Kit. KAT7/MYST2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13751-1-AP targets MYST2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 MYST2 fusion protein Ag4693 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MYST histone acetyltransferase 2
别名 KAT7, KAT 7, Histone acetyltransferase KAT7, HBOa, HBO1
计算分子量 611 aa, 71 kDa
观测分子量71 kDa
GenBank蛋白编号BC032640
基因名称 MYST2
Gene ID (NCBI) 11143
RRIDAB_2266703
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95251
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYST2, also named as HBO1, HBOa MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2, belongs to the MYST (SAS/MOZ) family. It specifically represses AR mediated transcription. MYST2 is a candidate oncogene. It enhances the anchorage-independent growth of breast cancer cells.(PMID:19372580) MYST2 is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. It is an important downstream molecule of ERa, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2.

实验方案

Product Specific Protocols
WB protocol for MYST2 antibody 13751-1-APDownload protocol
IHC protocol for MYST2 antibody 13751-1-APDownload protocol
IF protocol for MYST2 antibody 13751-1-APDownload protocol
IP protocol for MYST2 antibody 13751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Authors - Ling Li
  • KO Validated
View Article
humanWB

Adv Sci (Weinh)

Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells

Authors - Ling Li
View Article
humanWB,IF,IP

Autophagy

KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.

Authors - Guokai Yan
View Article
humanWB

Cancer Res

Hbo1 is a cyclin E/CDK2 substrate that enriches breast cancer stem-like cells.

Authors - Mylinh T Duong
View Article
humanWB

Mol Cell Proteomics

Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).

Authors - Heguo Yu
View Article
humanWB

Mol Cell Proteomics

Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human.

Authors - Ding Wang
View Article