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MYT1 Polyclonal antibody

MYT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26204-1-AP

Print datasheet

Synonyms

MYTI, Myelin transcription factor I, MTF1, KIAA1050, KIAA0835

验证数据展示

  • WB analysis of SH-SY5Y using 26204-1-AP

    WB analysis of SH-SY5Y using 26204-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 26204-1-AP (MYT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 26204-1-AP

    IHC staining of mouse brain using 26204-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26204-1-AP (MYT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 26204-1-AP

    IHC staining of mouse brain using 26204-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26204-1-AP (MYT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 26204-1-AP

    IHC staining of rat brain using 26204-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26204-1-AP (MYT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26204-1-AP targets MYT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MYT1 fusion protein Ag24580 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myelin transcription factor 1
Synonyms MYTI, Myelin transcription factor I, MTF1, KIAA1050, KIAA0835
Calculated Molecular Weight591aa,65 kDa; 1121aa,122 kDa
Observed Molecular Weight 122 kDa
GenBank Accession NumberBC062313
Gene Symbol MYT1
Gene ID (NCBI) 4661
RRIDAB_2880424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01538
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYT1 antibody 26204-1-APDownload protocol
IHC protocol for MYT1 antibody 26204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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