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验证数据展示

  • IHC staining of human breast cancer using 66237-1-Ig

    IHC staining of human breast cancer using 66237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66237-1-Ig (Mammaglobin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Mammaglobin A antibody included
  • IHC staining of human breast cancer using 66237-1-Ig

    IHC staining of human breast cancer using 66237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66237-1-Ig (Mammaglobin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Mammaglobin A antibody included
  • IF Staining of human breast cancer using 66237-1-Ig

    IF Staining of human breast cancer using 66237-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Mammaglobin A antibody (66237-1-Ig, Clone: 2E5D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Mammaglobin A Monoclonal antibody

Mammaglobin A Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 66237-1-Ig

Print datasheet

CloneNo. 2E5D4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, ELISA

Synonyms

Mammaglobin 1, Mammaglobin A, MGB1, SCGB2A2, UGB2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy Mammaglobin A Ready-To-Use IHC Kit. Mammaglobin A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66237-1-Ig targets Mammaglobin A in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen Mammaglobin A fusion protein Ag22485 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name secretoglobin, family 2A, member 2
Synonyms Mammaglobin 1, Mammaglobin A, MGB1, SCGB2A2, UGB2
Calculated Molecular Weight 93 aa, 10 kDa
GenBank Accession NumberBC128252
Gene Symbol Mammaglobin A
Gene ID (NCBI) 4250
RRIDAB_2881626
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13296
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammaglobin A, also known as SCGB2A2, is a member of the secretoglobin superfamily. Mammaglobin A is a breast cancer-associated antigen almost exclusively over-expressed in primary and metastatic human breast cancers, making it a specific molecular marker and a potential therapeutic target for breast cancer. This monoclonal antibody is raised against full-length of 93-amino acid human mammaglobin A.

实验方案

Product Specific Protocols
IHC protocol for Mammaglobin A antibody 66237-1-IgDownload protocol
IF protocol for Mammaglobin A antibody 66237-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols