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Maspin Monoclonal antibody, PBS Only

Maspin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G5A3

Cat no : 67633-1-PBS

Print datasheet

Synonyms

maspin, Peptidase inhibitor 5, PI 5, PI5, Serpin B5, SERPINB5, SPB5

验证数据展示

  • WB analysis using 67633-1-Ig (same clone as 67633-1-PBS)

    WB analysis using 67633-1-Ig (same clone as 67633-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67633-1-Ig (Maspin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67633-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 67633-1-Ig (same clone as 67633-1-PBS)

    IHC staining of human oesophagus cancer using 67633-1-Ig (same clone as 67633-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67633-1-Ig (Maspin antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67633-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 67633-1-Ig (same clone as 67633-1-PBS)

    IHC staining of human oesophagus cancer using 67633-1-Ig (same clone as 67633-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67633-1-Ig (Maspin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67633-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 67633-1-Ig (same clone as 67633-1-PBS)

    IF Staining of HEK-293 using 67633-1-Ig (same clone as 67633-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Maspin antibody (67633-1-Ig, Clone: 3G5A3 ) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67633-1-PBS in a different storage buffer formulation.

产品信息

67633-1-PBS targets Maspin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Maspin fusion protein Ag19129 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 5
Synonyms maspin, Peptidase inhibitor 5, PI 5, PI5, Serpin B5, SERPINB5, SPB5
Calculated Molecular Weight 375 aa, 42 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC020713
Gene Symbol Maspin
Gene ID (NCBI) 5268
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36952
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SERPINB5, also named as PI-5, Maspin and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.