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验证数据展示

  • IHC staining of human spleen using 15825-1-AP

    IHC staining of human spleen using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MCL1 antibody included
  • IHC staining of human spleen using 15825-1-AP

    IHC staining of human spleen using 15825-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15825-1-AP (MCL1L-specific antibody at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with MCL1 antibody included

MCL1L-Specific Polyclonal antibody

MCL1L-Specific Polyclonal Antibody for IHC, ELISA
Cat No. 15825-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA

别名

MCL1, Mcl 1, Induced myeloid leukemia cell differentiation protein Mcl-1, EAT, Bcl-2-related protein EAT/mcl1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman spleen tissue, human brain tissue, human breast cancer tissue, human cervical cancer tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human testis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MCL1 Ready-To-Use IHC Kit. MCL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15825-1-AP targets MCL1L-Specific in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myeloid cell leukemia sequence 1 (BCL2-related)
别名 MCL1, Mcl 1, Induced myeloid leukemia cell differentiation protein Mcl-1, EAT, Bcl-2-related protein EAT/mcl1
计算分子量 37 kDa
GenBank蛋白编号NM_182763
基因名称 MCL1
Gene ID (NCBI) 4170
RRIDAB_2144112
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ07820
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. This antibody is specific to MCL1L isoform.

实验方案

Product Specific Protocols
IHC protocol for MCL1L-Specific antibody 15825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols