验证数据展示
经过测试的应用
| Positive WB detected in | LPS and Brefeldin A treated RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26161-1-AP targets MCP-1/CCL2 in WB, IHC, IF, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | mouse |
| 文献引用反应性 | mouse, rat |
| 免疫原 | MCP-1/CCL2 fusion protein Ag24085 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) ligand 2 |
| 别名 | Ccl2, MCP1, MCP-1, Chemokine (CC motif) ligand 2, chemokine (C C motif) ligand 2 |
| 观测分子量 | 16-20 kDa |
| GenBank蛋白编号 | NM-011333 |
| 基因名称 | MCP-1/CCL2 |
| Gene ID (NCBI) | 20296 |
| RRID | AB_2918100 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MCP-1/CCL2 antibody 26161-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Pharmacol Sin Macrophages promote the transition from myocardial ischemia reperfusion injury to cardiac fibrosis in mice through GMCSF/CCL2/CCR2 and phenotype switching | ||
Int J Mol Sci Enhanced Activation of the S1PR2-IL-1β-Src-BDNF-TrkB Pathway Mediates Neuroinflammation in the Hippocampus and Cognitive Impairment in Hyperammonemic Rats | ||
Front Cell Neurosci Effects of Ischemia on the Migratory Capacity of Microglia Along Collagen Microcontact Prints on Organotypic Mouse Cortex Brain Slices. | ||
Pharmaceutics Targeted Therapy of Acute Liver Injury via Cryptotanshinone-Loaded Biomimetic Nanoparticles Derived from Mesenchymal Stromal Cells Driven by Homing | ||
J Biol Chem rhFGF-21 accelerates corneal epithelial wound healing through the attenuation of oxidative stress and inflammatory mediators in diabetic mice | ||
Proc Natl Acad Sci U S A Bacterial indole-3-lactic acid affects epithelium-macrophage crosstalk to regulate intestinal homeostasis |