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Mouse MCP-1/CCL2 Recombinant antibody, PBS Only (Capture/Detector)

MCP-1/CCL2 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230483H3

Cat no : 83157-4-PBS

Print datasheet

Synonyms

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

验证数据展示

  • Sandwich ELISA standard curve of MP00168-4

    Sandwich ELISA standard curve of MP00168-4

    Sandwich ELISA standard curve of MP00168-4, Mouse MCP-1 Recombinant Matched Antibody Pair - PBS only. 83157-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0427. 83157-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00168-4

    Sample test of MP00168-4

    J774A.1 mouse reticulum cell sarcoma macrophage cells (1 x 10*6 cells/mL) were cultured for 3 days in DMEM containing 10% fetal bovine serum. Cells were untreated or treated with 500 ng/mL recombinant mouse IFN-γ and 10 ng/mL LPS. An aliquot of the cell culture supernatants were removed, assayed for mouse MCP-1, and measured 6,956.6 pg/mL and 58,869.9 pg/mL.

  • Sample test of MP00168-4

    Sample test of MP00168-4

    Serum of sixteen mice was measured. The MCP-1 concentration of detected samples was determined to be 77.0 pg/mL with a range of 39.7 - 135.3 pg/mL.

  • Cytometric bead array standard curve of MP00168-1

    Cytometric bead array standard curve of MP00168-1

    Cytometric bead array standard curve of MP00168-1, Mouse Ccl2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83157-4-PBS. Detection antibody: 83157-2-PBS. Standard: Eg0427. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00168-3

    Cytometric bead array standard curve of MP00168-3

    Cytometric bead array standard curve of MP00168-3, Mouse Ccl2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83157-4-PBS. Detection antibody: 83157-1-PBS. Standard: Eg0427. Range: 1.25-20 ng/mL.

产品信息

83157-4-PBS targets MCP-1/CCL2 as part of a matched antibody pair:

MP00168-1: 83157-4-PBS capture and 83157-2-PBS detection (validated in Cytometric bead array)

MP00168-3: 83157-4-PBS capture and 83157-1-PBS detection (validated in Cytometric bead array)

MP00168-4: 83157-6-PBS capture and 83157-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 2
Synonyms Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2
GenBank Accession NumberNM-011333
Gene Symbol MCP-1/CCL2
Gene ID (NCBI) 20296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C