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验证数据展示

  • WB analysis of RAW 264.7 using 83157-5-RR (same clone as 83157-5-PBS)

    WB analysis of RAW 264.7 using 83157-5-RR (same clone as 83157-5-PBS)

    Un-treated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83157-5-RR (Mcp1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83157-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83157-5-RR

    Affinity and Kinetic Characterization of 83157-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83157-5-RR against Mouse MCP-1 were performed. The affinity constant is 60.5 pM.

Mouse MCP-1/CCL2 Recombinant antibody, PBS Only

MCP-1/CCL2 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 83157-5-PBS

产品说明书

CloneNo. 230483F11

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, Indirect ELISA

别名

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83157-5-PBS targets MCP-1/CCL2 in WB, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) ligand 2
别名 Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2
观测分子量16 kDa
GenBank蛋白编号NM-011333
基因名称 MCP-1/CCL2
Gene ID (NCBI) 20296
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.