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Mouse MCP-1/CCL2 Recombinant antibody, PBS Only

MCP-1/CCL2 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230483F11

Cat no : 83157-5-PBS

Print datasheet

Synonyms

Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2

验证数据展示

  • WB analysis of RAW 264.7 using 83157-5-RR (same clone as 83157-5-PBS)

    WB analysis of RAW 264.7 using 83157-5-RR (same clone as 83157-5-PBS)

    Un-treated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83157-5-RR (Mcp1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83157-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83157-5-RR

    Affinity and Kinetic Characterization of 83157-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83157-5-RR against Mouse MCP-1 were performed. The affinity constant is 60.5 pM.

产品信息

83157-5-PBS targets MCP-1/CCL2 in WB, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 2
Synonyms Ccl2, MCP1, MCP-1, chemokine (C C motif) ligand 2, CCL-2
Observed Molecular Weight16 kDa
GenBank Accession NumberNM-011333
Gene Symbol MCP-1/CCL2
Gene ID (NCBI) 20296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.