验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, mouse stomach tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IF/ICC detected in | C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51027-2-AP targets MECR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, yeast |
| 免疫原 | MECR fusion protein Ag0465 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitochondrial trans-2-enoyl-CoA reductase |
| 别名 | Nuclear receptor-binding factor 1, NRBF-1, Nrbf1, Enoyl-[acyl-carrier-protein] reductase, mitochondrial, EC:1.3.1.104 |
| 计算分子量 | 40 kDa |
| 观测分子量 | 37-40 kDa |
| GenBank蛋白编号 | BC003864 |
| 基因名称 | MECR |
| Gene ID (NCBI) | 26922 |
| RRID | AB_615013 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9DCS3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mitochondrial Trans-2-Enoyl-CoA Reductase is abbrivated as MECR, involved in human mitochonrial fatty acid synthesis(mtFAS) as an oxidoreductase. (PMID: 27817865) MECR has molecular weight can be calculated around 37KDa. With immunopercipitation reaction on mouse heart samples, the IgG heavy chain, dimer of MECR is around 65KDa. Numerous studies have shown disorder of lipid metabolism is closely related with malignance, especially in liver cancer. As futher research suggests that MECR has close realitionship with cell prolifereation, appotosis, and metastasis (PMID: 31178528)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MECR antibody 51027-2-AP | Download protocol |
| IF protocol for MECR antibody 51027-2-AP | Download protocol |
| IP protocol for MECR antibody 51027-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Hum Genet MECR Mutations Cause Childhood-Onset Dystonia and Optic Atrophy, a Mitochondrial Fatty Acid Synthesis Disorder. | ||
Elife Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria. | ||
J Neurosci Impaired mitochondrial fatty acid synthesis leads to neurodegeneration in mice. | ||
Hum Mol Genet Genetic modifications of Mecr reveal a role for mitochondrial 2-enoyl-CoA/ACP reductase in placental development in mice. | ||
Nat Commun An engineered variant of MECR reductase reveals indispensability of long-chain acyl-ACPs for mitochondrial respiration | ||
Lipids Health Dis A novel lipid metabolism-based risk model associated with immunosuppressive mechanisms in diffuse large B-cell lymphoma |