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Midkine Polyclonal antibody

Midkine Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28546-1-AP

Print datasheet

Synonyms

ARAP, FLJ27379, MDK, Midkine, MK, MK1, NEGF2

验证数据展示

  • WB analysis using 28546-1-AP

    WB analysis using 28546-1-AP

    Mouse embryo tissues were subjected to SDS PAGE followed by western blot with 28546-1-AP (Midkine antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 28546-1-AP

    IHC staining of human pancreas cancer using 28546-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MDK antibody included
  • IHC staining of human pancreas cancer using 28546-1-AP

    IHC staining of human pancreas cancer using 28546-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28546-1-AP (Midkine antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MDK antibody included

经过测试的应用

Positive WB detected inmouse embryo tissue
Positive IHC detected inhuman pancreas cancer tissue, human stomach cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MDK Ready-To-Use IHC Kit. MDK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28546-1-AP targets Midkine in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse
Cited Reactivitymouse
Immunogen Midkine fusion protein Ag29185 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name midkine (neurite growth-promoting factor 2)
Synonyms ARAP, FLJ27379, MDK, Midkine, MK, MK1, NEGF2
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC011704
Gene Symbol MDK
Gene ID (NCBI) 4192
RRIDAB_2881168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21741
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Midkine antibody 28546-1-APDownload protocol
IHC protocol for Midkine antibody 28546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

EBioMedicine

Spatial and single-cell colocalisation analysis reveals MDK-mediated immunosuppressive environment with regulatory T cells in colorectal carcinogenesis

Authors - Masahiro Hashimoto
View Article