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Mlkl Polyclonal antibody

Mlkl Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30145-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 30145-1-AP

    WB analysis using 30145-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30145-1-AP (Mlkl antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30145-1-AP

    WB analysis using 30145-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30145-1-AP (Mlkl antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 30145-1-AP

    IF Staining of NIH/3T3 using 30145-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Mlkl antibody (30145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 30145-1-AP

    IF Staining of HepG2 using 30145-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Mlkl antibody (30145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected in4T1 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inNIH/3T3 cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

30145-1-AP targets Mlkl in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Mlkl fusion protein Ag32514 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mixed lineage kinase domain-like
Synonyms
Calculated Molecular Weight54kd
Observed Molecular Weight 54 kDa
GenBank Accession NumberNM_001310613
Gene Symbol Mlkl
Gene ID (NCBI) 74568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9D2Y4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mixed lineage kinase domain-like pseudokinase (Mlkl) belongs to the protein kinase superfamily and has two MWs of 54 and 30 kDa. Mlkl plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.

实验方案

Product Specific Protocols
WB protocol for Mlkl antibody 30145-1-APDownload protocol
IF protocol for Mlkl antibody 30145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

FASEB J

Metformin switches cell death modes to soothe the apical periodontitis via ZBP1

Authors - Hui Liu
View Article