验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, mouse spleen tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IF/ICC detected in | U2OS cells, A431 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
30592-1-AP targets Mmp9 in WB, IF/ICC, IP, ELISA applications and shows reactivity with mouse, human samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | mouse, human |
| Cited Reactivity | human, mouse |
| Immunogen | Recombinant protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | matrix metallopeptidase 9 |
| Synonyms | AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9, MMP 9, Mmp9, pro MMP 9 |
| Calculated Molecular Weight | 81kd |
| Observed Molecular Weight | 80-100 kDa |
| GenBank Accession Number | NM_013599 |
| Gene Symbol | Mmp9 |
| Gene ID (NCBI) | 17395 |
| RRID | AB_3086370 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41245-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The calculated molecular weight of Mmp9 is 80 kDa, the pro-MMP9 is 92 kDa, and this protein can exist as a dimer of 180 kDa (PMID:7492685).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Mmp9 antibody 30592-1-AP | Download protocol |
| IF protocol for Mmp9 antibody 30592-1-AP | Download protocol |
| IP protocol for Mmp9 antibody 30592-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Small A Constant Filgotinib Delivery Adhesive Platform Based on Polyethylene Glycol (PEG) Hydrogel for Accelerating Wound Healing via Restoring Macrophage Mitochondrial Homeostasis | ||
Oncol Lett Transcription factor ETS1‑mediated ECT2 expression promotes the malignant behavior of prostate cancer cells | ||
J Inflamm Res A Neutrophil Extracellular Traps-Related Gene Trait Revealed the Prospective Therapy Strategy of Coronary Atherosclerosis |