• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Mmp9 Polyclonal antibody

Mmp9 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 30592-1-AP

Print datasheet

Synonyms

AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9, MMP 9, Mmp9, pro MMP 9

验证数据展示

  • WB analysis using 30592-1-AP

    WB analysis using 30592-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30592-1-AP (Mmp9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 30592-1-AP

    IP experiment of mouse lung using 30592-1-AP

    IP result of anti-Mmp9 (IP:30592-1-AP, 4ug; Detection:30592-1-AP 1:6000) with mouse lung tissue lysate 1200 ug.

  • IF Staining of A431 using 30592-1-AP

    IF Staining of A431 using 30592-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of U2OS using 30592-1-AP

    IF Staining of U2OS using 30592-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse lung tissue, mouse spleen tissue
Positive IP detected inmouse lung tissue
Positive IF/ICC detected inU2OS cells, A431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

30592-1-AP targets Mmp9 in WB, IF/ICC, IP, ELISA applications and shows reactivity with mouse, human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity mouse, human
Cited Reactivityhuman
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix metallopeptidase 9
Synonyms AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9, MMP 9, Mmp9, pro MMP 9
Calculated Molecular Weight81kd
Observed Molecular Weight80-100 kDa
GenBank Accession NumberNM_013599
Gene Symbol Mmp9
Gene ID (NCBI) 17395
RRIDAB_3086370
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41245-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The calculated molecular weight of Mmp9 is 80 kDa, the pro-MMP9 is 92 kDa, and this protein can exist as a dimer of 180 kDa (PMID:7492685).

实验方案

Product Specific Protocols
WB protocol for Mmp9 antibody 30592-1-APDownload protocol
IF protocol for Mmp9 antibody 30592-1-APDownload protocol
IP protocol for Mmp9 antibody 30592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncol Lett

Transcription factor ETS1‑mediated ECT2 expression promotes the malignant behavior of prostate cancer cells

Authors - Bo Zheng
View Article
humanWB

J Inflamm Res

A Neutrophil Extracellular Traps-Related Gene Trait Revealed the Prospective Therapy Strategy of Coronary Atherosclerosis

Authors - Zhetao Li
View Article