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验证数据展示

  • WB analysis using 82009-1-RR (same clone as 82009-1-PBS)

    WB analysis using 82009-1-RR (same clone as 82009-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82009-1-RR (MSN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82009-1-RR (same clone as 82009-1-PBS)

    WB analysis of HeLa using 82009-1-RR (same clone as 82009-1-PBS)

    WB result of Moesin antibody (82009-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Moesin transfected HeLa cells. This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 82009-1-RR (same clone as 82009-1-PBS)

    IHC staining of human placenta using 82009-1-RR (same clone as 82009-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSN antibody included
  • IHC staining of mouse kidney using 82009-1-RR (same clone as 82009-1-PBS)

    IHC staining of mouse kidney using 82009-1-RR (same clone as 82009-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSN antibody included
  • IHC staining of mouse kidney using 82009-1-RR (same clone as 82009-1-PBS)

    IHC staining of mouse kidney using 82009-1-RR (same clone as 82009-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSN antibody included
  • IF Staining of HepG2 using 82009-1-RR (same clone as 82009-1-PBS)

    IF Staining of HepG2 using 82009-1-RR (same clone as 82009-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Moesin antibody (82009-1-RR, Clone: 3B8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82009-1-RR

    Affinity and Kinetic Characterization of 82009-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82009-1-RR against Human MSN were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

Moesin Recombinant antibody, PBS Only

Moesin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82009-1-PBS

产品说明书

CloneNo. 3B8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MSN, Membrane-organizing extension spike protein, 3B8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MSN Ready-To-Use IHC Kit. MSN primary antibody included.

产品信息

82009-1-PBS targets Moesin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Moesin fusion protein Ag9623 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 moesin
别名 MSN, Membrane-organizing extension spike protein, 3B8
计算分子量 577 aa, 68 kDa
观测分子量 68-70 kDa
GenBank蛋白编号BC017293
基因名称 Moesin
Gene ID (NCBI) 4478
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP26038
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.