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  • Featured Product
  • KD/KO Validated

Moesin Recombinant antibody, PBS Only

Moesin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B8

Cat no : 82009-1-PBS

Print datasheet

Synonyms

MSN, Membrane-organizing extension spike protein, 3B8

验证数据展示

  • WB analysis using 82009-1-RR (same clone as 82009-1-PBS)

    WB analysis using 82009-1-RR (same clone as 82009-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82009-1-RR (MSN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82009-1-RR (same clone as 82009-1-PBS)

    WB analysis of HeLa using 82009-1-RR (same clone as 82009-1-PBS)

    WB result of Moesin antibody (82009-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Moesin transfected HeLa cells. This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 82009-1-RR (same clone as 82009-1-PBS)

    IHC staining of human placenta using 82009-1-RR (same clone as 82009-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSN antibody included
  • IHC staining of mouse kidney using 82009-1-RR (same clone as 82009-1-PBS)

    IHC staining of mouse kidney using 82009-1-RR (same clone as 82009-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSN antibody included
  • IHC staining of mouse kidney using 82009-1-RR (same clone as 82009-1-PBS)

    IHC staining of mouse kidney using 82009-1-RR (same clone as 82009-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSN antibody included
  • IF Staining of HepG2 using 82009-1-RR (same clone as 82009-1-PBS)

    IF Staining of HepG2 using 82009-1-RR (same clone as 82009-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Moesin antibody (82009-1-RR, Clone: 3B8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82009-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82009-1-RR

    Affinity and Kinetic Characterization of 82009-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82009-1-RR against Human MSN were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy MSN Ready-To-Use IHC Kit. MSN primary antibody included.

产品信息

82009-1-PBS targets Moesin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Moesin fusion protein Ag9623 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name moesin
Synonyms MSN, Membrane-organizing extension spike protein, 3B8
Calculated Molecular Weight 577 aa, 68 kDa
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC017293
Gene Symbol Moesin
Gene ID (NCBI) 4478
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26038
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.