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验证数据展示

  • WB analysis using 68825-1-Ig

    WB analysis using 68825-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68825-1-Ig (Histone H3.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Mono/Di-Methyl-Histone H3 (Lys9) Monoclonal antibody

Mono/Di-Methyl-Histone H3 (Lys9) Monoclonal Antibody for WB, ELISA
Cat No. 68825-1-Ig

产品说明书

CloneNo. 2E6D11

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, LNCaP cells, Jurkat cells, MOLT-4 cells, K-562 cells, NIH/3T3 cells, C2C12 cells, HSC-T6 cells
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68825-1-Ig targets Mono/Di-Methyl-Histone H3 (Lys9) in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 histone cluster 1, H3a
别名 H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
观测分子量17 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3670436
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression. This antibody is specific to monomethyl or dimethyl-Histone H3 while it does not recognize trimethyl-Histone H3 (Lys9). It is also named as H3K9me1/2.

实验方案

Product Specific Protocols
WB protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 68825-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols