验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, HeLa cells, THP-1 cells, K-562 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
Positive Dot Blot detected in | peptide |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
DOT BLOT | DOT BLOT : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82825-2-RR targets Mono-Methyl-Histone H3 (Lys36) in WB, IF/ICC, FC (Intra), Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, FC (Intra), Dot Blot, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | histone cluster 1, H3a |
Synonyms | HIST1H3A, 241138B6, Histone-H3, HistoneH3, Histone H3 |
Observed Molecular Weight | 15 kDa |
GenBank Accession Number | BC066245 |
Gene Symbol | HIST1H3A |
Gene ID (NCBI) | 8350 |
RRID | AB_3670565 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P68431 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Mono-Methyl-Histone H3 (Lys36) antibody 82825-2-RR | Download protocol |
IF protocol for Mono-Methyl-Histone H3 (Lys36) antibody 82825-2-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |