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验证数据展示

  • Dot Blot experiment of peptide using 84740-1-RR

    Dot Blot experiment of peptide using 84740-1-RR

    Dot blot analysis was used to confirm the specificity of 84740-1-RR Mono-Methyl-Histone H3 (Lys4) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • WB analysis using 84740-1-RR

    WB analysis using 84740-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84740-1-RR (Mono-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Mono-Methyl-Histone H3 (Lys4) Recombinant antibody

Mono-Methyl-Histone H3 (Lys4) Recombinant Antibody for WB, Dot Blot, ELISA
Cat No. 84740-1-RR

产品说明书

CloneNo. 241940G8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Dot Blot, ELISA

别名

H3K4Me1, H3K4Me, Me Histone H3, Me Histone H3 (Lys4), Me Histone H3 Lys4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, U-87 MG cells, NIH/3T3 cells, mouse liver tissue, mouse brain tissue
Positive Dot Blot detected inpeptide
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84740-1-RR targets Mono-Methyl-Histone H3 (Lys4) in WB, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Dot Blot, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H3a
别名 H3K4Me1, H3K4Me, Me Histone H3, Me Histone H3 (Lys4), Me Histone H3 Lys4
观测分子量15 kDa
GenBank蛋白编号BC066245
基因名称 HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3672125
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP68431
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Mono-Methyl-Histone H3 (Lys4) antibody 84740-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols