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Monochlorobimane

Monochlorobimane (mBBr) 是一种荧光染料 ,λex 值为380 nm，λem 值为470 nm。Monochlorobimane 可用于测定细胞中谷胱甘肽 (GSH)。

CAS号

76421-73-3

分子式

C10H11ClN2O2

主要靶点

仅限科研使用

Cat No : CM09609

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Synonyms

mBBr|Chlorobimane

验证数据展示

  • CAS No.: 76421-73-3

    Monochlorobimane
    CAS#: 76421-73-3

产品信息

Monochlorobimane is a fluorescent dye with a λex value of 380 nm and a λem value of 470 nm.Monochlorobimane can be used to measure glutathione (GSH) in cells.

CAS号 76421-73-3
分子式 C10H11ClN2O2
主要靶点
主要通路
分子量 226.66
纯度 ， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名 mBBr|Chlorobimane

体外活性

A common technique to measure Glutathione (GSH) in cultured cells is to add Monochlorobimane to the culture medium where it readily enters cells to form a fluorescent GSH-monochlorobimane adduct that can be measured fluorometrically. The Monochlorobimane approach gives comparable results to the HPLC technique. Monochlorobimane concentration can be lowered to 25 mM and still accurately measure homogenate GSH content.[1]

溶解度

DMSO:45.0 mg/mL (198.5 mM),Sonification is recommended.

参考文献

1.Kamencic H, et al. Monochlorobimane fluorometric method to measure tissue glutathione. Anal Biochem. 2000 Nov 1;286(1):35-7.
2.Machado M D, et al. Assessment of cellular reduced glutathione content in Pseudokirchneriella subcapitata using monochlorobimane. Journal of applied phycology. 2012; 24: 1509-1516.
3.Hohwy M, et al. Novel prostaglandin D synthase inhibitors generated by fragment-based drug design. J Med Chem. 2008;51(7):2178-2186.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
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C1   V1   C2   V2