Mouse AFP ELISA Kit
Sensitivity
8.9 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10092 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse AFP ELISA kit is to be used to detect and quantify protein levels of endogenous mouse AFP. The assay recognizes mouse AFP. An antibody specific for mouse AFP has been pre-coated onto the microwells. The mouse AFP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse AFP is added to detect the captured mouse AFP protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse AFP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 8.9 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11576 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 86% | 69%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,542.0 | 110.5 | 4.3 |
| 2 | 20 | 624.6 | 18.5 | 3.0 |
| 3 | 20 | 152.1 | 5.7 | 3.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,531.3 | 121.8 | 4.8 |
| 2 | 24 | 646.8 | 42.5 | 6.6 |
| 3 | 24 | 165.0 | 14.2 | 8.6 |
Background Information
AFP (alpha-fetoprotein) is a major plasma protein found in the fetus while plasma AFP level is decreased rapidly after birth. AFP is a vital marker of the hepatocyte lineage. High AFP concentrations is related with tumor cell growth. Detection of AFP in plasma is important in diagnosis of hepatocellular carcinoma (HCC), stomach cancer and germ cell cancers. Altered levels of both fetal and maternal AFP are associated with hypothyroidism, autoimmune disorders, and heart defects. Measurement of AFP in maternal blood or amniotic fluid of pregnant women is used in screening developmental abnormalities such as aneuploidy. And serum AFP level is elevated in people with developmental birth defects and neurologic disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Afp, alpha fetoprotein |