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Recombinant Mouse CD45/PTPRC (His Tag)

ED50

/

Species

Mouse

Purity

>90 %, SDS-PAGE

GeneID

19264

Accession

P06800-4

Cat No : Eg0845

Print datasheet

Synonyms

B220, Cd45, LCA, Leukocyte common antigen, Ptprc

验证数据展示

  • Purity of Recombinant Mouse CD45/PTPRC was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD45/PTPRC was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
/
Source CHO-derived Mouse CD45/PTPRC protein Gln26-Lys566 (Accession# P06800-4) with a His Tag at the C-terminus.
Predicted Molecular Mass 59.5 kDa
SDS-PAGE 100-150 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets. CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling.

References:

1. F W Shen, et al. (1986) Immunogenetics. 24(3):146-9.
2. J G Altin, et al. (1997) Review Immunol Cell Biol. 75(5):430-45.
3. Nicholas D Huntington, et al. (2006) Nat Immunol. 7(2):190-8. 
4. Salema Jafri, et al. (2017) Sci Rep. 7(1):3495.