Mouse CD68 ELISA Kit
Sensitivity
0.7 pg/mL
Range
7.8-500 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10112 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD68 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD68. The assay recognizes mouse CD68. An antibody specific for mouse CD68 has been pre-coated onto the microwells. The mouse CD68 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD68 is added to detect the captured mouse CD68 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD68 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatant，Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.7 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12514 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 89% | 78%-101% |
| Cell lysate | 99% | 92%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 263.1 | 8.9 | 3.4 |
| 2 | 20 | 63.0 | 3.5 | 5.6 |
| 3 | 20 | 15.9 | 1.2 | 7.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 266.1 | 21.9 | 8.2 |
| 2 | 24 | 61.9 | 5.2 | 8.4 |
| 3 | 24 | 13.7 | 0.7 | 5.3 |
Background Information
CD68 is a 110 kDa transmembrane glycoprotein widely expressed in monocyte cell types, such as macrophages, microglia, and osteoclasts. It is located at the lysosome and plasma membrane and is essential in various physiological and pathological processes, including atherosclerosis formation, inflammation and auto-immunity, bone-resorbing promotion, and tumor progression. It participates in the cellular response to lipopolysaccharide/oxidized low-density lipoprotein particle stimulus and negative regulation of dendritic cell antigen processing and presentation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cd68, CD68 antigen, gp110, Scard1 |