Mouse CX3CL1 ELISA Kit
Sensitivity
0.09 ng/mL
Range
0.625-20 ng/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10076 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CX3CL1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CX3CL1. The assay recognizes mouse CX3CL1. An antibody specific for mouse CX3CL1 has been pre-coated onto the microwells. The mouse CX3CL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse CX3CL1 is added to detect the captured mouse CX3CL1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CX3CL1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants,Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 0.09 ng/mL |
| Range | 0.625-20 ng/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20312 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysates | 87% | 77%-97% |
| Mouse serum | 96% | 78%-111% |
| Cell culture supernates | 95% | 79%-111% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 9.63 | 0.41 | 4.29 |
| 2 | 20 | 2.34 | 0.13 | 5.70 |
| 3 | 20 | 1.27 | 0.03 | 2.54 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 9.64 | 0.37 | 3.89 |
| 2 | 24 | 2.40 | 0.09 | 3.66 |
| 3 | 24 | 1.23 | 0.08 | 6.47 |
Background Information
Chemokines direct the trafficking of white blood cells in immune surveillance, playing a key role in inflammatory and infectious diseases such as AIDS. CX3CL1, also known as fractalkine (FKN), is a unique, membrane-bound chemokine, with a transmembrane domain and the chemokine domain on top of a long mucinlike stalk. This protein can exist in two forms, a membrane-bound form or a shed soluble form, after extracellular proteolysis at a membrane-proximal dibasic cleavage site. The soluble CX3CL1 has potent chemoattractant activity for T cells and monocytes, and the cell-surface-bound protein, which is induced by primary proinflammatory signals in activated primary endothelial cells, promotes strong adhesion of those leukocytes. Presence of CX3CL1 and its receptor CX3CR1 in lymphocytes and their enhanced cellular expression during inflammatory conditions suggests that the CX3CL1-CX3CR1 duo participates in immune-related inflammatory diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AB030188, ABCD 3, AI848747, CX3C, Cx3cl1, Cxc3, D8Bwg0439e, Scyd1 |