Mouse Chemerin ELISA Kit
Sensitivity
1.9 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10074 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse Chemerin ELISA kit is to be used to detect and quantify protein levels of endogenous mouse Chemerin. The assay recognizes mouse Chemerin. An antibody specific for mouse Chemerin has been pre-coated onto the microwells. The mouse Chemerin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse Chemerin is added to detect the captured mouse Chemerin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse Chemerin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates |
| Assay type | Sandwich |
| Sensitivity | 1.9 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 71660 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 93% | 80%-109% |
| Cell culture supernatants | 110% | 82%-125% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 472.1 | 16.3 | 3.5 |
| 2 | 20 | 116.8 | 3.7 | 3.2 |
| 3 | 20 | 30.7 | 0.8 | 2.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 455.6 | 22.1 | 4.9 |
| 2 | 24 | 111.9 | 3.5 | 3.2 |
| 3 | 24 | 31.0 | 1.0 | 3.3 |
Background Information
Chemerin, also named as TIG2 and RARRES2, is adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). It positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. Chemerin have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 0610007L05Rik, AI303516, Rarres2 |