Mouse Control qPCR Primer Sets

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Mouse-Control-qPCR-Primer-Sets-AM765

Synonyms

chip, chromatin IP, chromatin immunoprecipitation, controls, PCR primers, primer sets, qPCR, endpoint PCR, end-point, mouse, positive control, Grik3 intron 8, 71021


Name Format Cat No. Price

Overview

Mouse Control qPCR Primer Sets were designed to serve as positive or negative ChIP controls when performing chromatin immunoprecipitation with mouse samples. Each primer set is a mixture of forward and reverse primers that have been validated for qPCR and endpoint PCR of ChIP samples from multiple mouse cell lines.

Click the Mouse qPCR Primer Sets tab for information on the target of each primer set and what it is an appropriate control for.

Mouse qPCR Primer Sets

Mouse Chromatin IP Control qPCR Primer Sets

Cat. No.   Name Gene Control for...
71011 Mouse Negative Control Primer Set 1 Gene desert on chromosome 6 Any transcription factor, LSD1, RNA pol II, 5-mC (MeDIP), H3K9ac, H3K14ac, H3K27ac, H3K4me1, H3K4me2, H3K4me3, H3K36me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac
71012 Mouse Negative Control Primer Set 2 Gene desert on chromosome 17 Any transcription factor, LSD1, RNA pol II, 5-mC (MeDIP), H3K9ac, H3K14ac, H3K27ac, H3K4me1, H3K4me2, H3K4me3, H3K36me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac
71013 Mouse Negative Control Primer Set 3 Actb promoter H3K27me3, EZH2
71014 Mouse Negative Control Primer Set 4 Gapdh promoter H3K27me3, EZH2
71015 Mouse Positive Control Primer Set Actb-1 Actb intron 3 RNA pol II phospho Ser2, H3K36me3
71016 Mouse Positive Control Primer Set Gapdh-1 Gapdh intron 2 RNA pol II phospho Ser2, H3K36me3
71017 Mouse Positive Control Primer Set Actb-2 Actb promoter H3K9ac, H3K14ac, H3K4me2, H3K4me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac, HDAC1, LSD1, Total RNA pol II, RNA pol II phospho Ser5
71018 Mouse Positive Control Primer Set Gapdh-2 Gapdh promoter H3K9ac, H3K14ac, H3K4me2, H3K4me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac, Total RNA pol II, RNA pol II phospho Ser5
71019 Mouse Positive Control Primer Set Hoxc10 Hoxc10 intron 1 H3K27me3, EZH2
71020 Mouse Positive Control Primer Set Pax-2 Region 5´ to Pax-2 H3K27me3,EZH2
71021 Mouse Positive Control Primer Set Grik3 Grik3 intron 8 5-mC (MeDIP)
71022 Mouse Positive Control Primer Set Zc3h13 Zc3h13 exon 10 5-mC (MeDIP)

 

Contents

Contents & Storage

Each control primer set contains both forward and reverse primers in 400 µl of nuclease-free distilled water. The final concentration for each primer is 2.5 µM.

The primer sets are shipped at room temperature and should be stored at -20°C upon receipt. The control primer sets are guaranteed stable for 6 months from date of receipt when stored properly.