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Recombinant Mouse CX3CL1 protein (His Tag)

ED50

/

Species

Mouse

Purity

>90 %, SDS-PAGE

GeneID

20312

Accession

O35188

Cat No : Eg0704

Print datasheet

Synonyms

Cx3cl1,ABCD 3,CX3C,Cxc3,Scyd1

验证数据展示

  • Purity of Recombinant Mouse CX3CL1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CX3CL1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Not tested
Source HEK293-derived Mouse CX3CL1 protein Gln25-Arg337 (Accession# O35188) with a His tag at the C-terminus.
Predicted Molecular Mass 34 kDa
SDS-PAGE 60-85 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CX3CL1, also known as fractalkine, is a trans-membrane chemokine that acts as a ligand for both CX3CR1 and integrins αvβ3 and α4β1. CX3CL1 is expressed by many hematopoietic and nonhematopoietic cells, including macrophages, endothelial cells, neurons, and fibroblasts. It is involved in immune responses and inflammation. CX3CL1 exists in a membrane-anchored molecule and a soluble form, generated by proteolytic cleavage.

References:

1. D L Rossi, et al. (1998) Genomics. 47(2):163-70.
2. K J Garton, et al. (2001) J Biol Chem. 276(41):37993-8001. 
3. Christian Hundhausen, et al. (2003) Blood. 102(4):1186-95.
4. Brian A Jones, et al. (2010) Mol Interv. 10(5):263-70. 
5. Masaaki Fujita, et al. (2012) J Immunol. 189(12):5809-19.