Mouse/Rat Flt-3 Ligand/FLT3L ELISA Kit

Sensitivity

3.0 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Mouse/Rat

Cat No : KE10072

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Synonyms

Flt3l, Flt3lg, Ly72L



Product Information

KE10072 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse/rat Flt-3 Ligand/FLT3L ELISA kit is to be used to detect and quantify protein levels of endogenous mouse/rat Flt-3 Ligand/FLT3L. The assay recognizes mouse/rat Flt-3 Ligand/FLT3L. An antibody specific for mouse/rat Flt-3 Ligand/FLT3L has been pre-coated onto the microwells. The mouse/rat Flt-3 Ligand/FLT3L protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse/rat Flt-3 Ligand/FLT3L is added to detect the captured mouse/rat Flt-3 Ligand/FLT3L protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse/Rat Flt-3 Ligand/FLT3L ELISA Kit
Tests 1 X 96 well plate
Sample type Mouse serum, Rat serum
Assay type Sandwich
Sensitivity 3.0 pg/mL
Range 15.6-1000 pg/mL
Reactivity Mouse/Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 14256

Recovery

Sample TypeAverageRange
Mouse serum103%95%-113%
Rat serum102%95%-112%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120503.916.03.2
220117.92.01.7
32029.41.75.7

InterAssay

Samplenmean ( pg/mL)SDCV%
124516.431.66.1
224121.05.64.6
32431.61.85.8

Background Information

Flt-3 Ligand, also known as FLT3L, is a cytokine that is of paramount importance in the proliferation of primitive hematopoietic progenitors. By acting on its receptor Flt-3 (CD135) and synergizing with other cytokines, FLT3L plays a pivotal role in promoting myeloid and lymphoid progenitors proliferation and differentiation. Besides regulating the development of immune cells during steady state conditions, FLT3L levels are raised upon inflammation. For example, increased serum levels of FLT3L has been observed during malaria in mice. Previous studies have shown that mice lacking FLT3L have deficient hematopoiesis function, resulting in reduced numbers of hematopoietic progenitor cells, dendritic cells, and natural killer cells.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Flt3l, Flt3lg, Ly72L