Mouse IL-6 ELISA Kit
Validation Data Gallery
Product Information
KE10091 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL-6 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-6. The assay recognizes mouse IL-6. An antibody specific for mouse IL-6 has been pre-coated onto the microwells. The mouse IL-6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse IL-6 is added to detect the captured mouse IL-6 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-6 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 4.0 pg/mL |
| Range | 12.5-800 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16193 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 88% | 84%-108% |
| Cell culture supernatants | 89% | 74%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 416.1 | 11.5 | 2.8 |
| 2 | 20 | 100.4 | 2.8 | 2.8 |
| 3 | 20 | 25.1 | 1.0 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 412.9 | 12.7 | 3.1 |
| 2 | 24 | 99.4 | 3.3 | 3.3 |
| 3 | 24 | 24.8 | 1.1 | 4.6 |
Background Information
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il 6, Il6, interleukin 6 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Adv Healthc Mater Enhanced Antibacterial And Wound Healing Using A Metal-Organic Cluster Inspired by Artificial Photosynthesis | ||
Int Immunopharmacol Eicosapentaenoic acid enhances the sensitivity of osteosarcoma to cisplatin by inducing ferroptosis through the DNA-PKcs/AKT/NRF2 pathway and reducing PD-L1 expression to attenuate immune evasion | ||
J Ethnopharmacol Guben Qingfei decoction attenuates LPS-induced acute lung injury by modulating the TLR4/NF-κB and Keap1/Nrf2 signaling pathways | ||
iScience The mechanisms and therapeutic potential of clopidogrel in mitigating diabetic cardiomyopathy in db/db mice | ||
Int J Biol Macromol Self-crosslinking hyaluronic acid hydrogel as an enteroprotective agent for the treatment of inflammatory bowel disease | ||
Pharmacol Res Amplifying hepatic L-aspartate levels suppresses CCl4-induced liver fibrosis by reversing glucocorticoid receptor β-mediated mitochondrial malfunction |