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Recombinant Mouse Neutrophil elastase protein (rFc Tag)

种属

Mouse

纯度

>90 %, SDS-PAGE

标签

rFc Tag

生物活性

未测试

Cat no : Eg2126

Print datasheet

Synonyms

EC:3.4.21.37, Ela2, Elane, Elastase-2, Leukocyte elastase

验证数据展示

  • Purity of Recombinant Mouse Neutrophil elastase was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Neutrophil elastase was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Mouse Neutrophil elastase protein Ser27-Arg260 (Accession# Q3UP87) with a rabbit IgG Fc tag at the C-terminus.
基因ID 50701
蛋白编号 Q3UP87
预测分子量 51.8 kDa
SDS-PAGE 55-65 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation. Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity, which is reported to cause mucin secretion (degranulation). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration. The full length protein has a signal peptide,propeptide and three glycosylation sites.

参考文献:

1.Kawabata K, Hagio T,Matsuoka S. (2002) Eur J Pharmacol. 451 (1):1-10.
2.Kohri K,Ueki IF,Nadel JA. (2002) Am J Physiol Lung Cell Mol Physiol. 283 (3):L531-40.
3.Carden, D et al.(1998) The American journal of physiology. 275, 2: H385-92.