Mouse PIGF-2 ELISA Kit
Sensitivity
0.7 pg/mL
Range
3.9-250 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10084 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse PIGF-2 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse PIGF-2.The assay recognizes mouse PIGF-2. An antibody specific for mouse PIGF-2 has been pre-coated onto the microwells. The mouse PIGF-2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse PIGF-2 is added to detect the captured mouse PIGF-2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse PIGF-2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.7 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 18654 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 101% | 91%-115% |
| Cell culture supernatants | 91% | 81%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 97.4 | 4.2 | 4.3 |
| 2 | 20 | 23.7 | 0.5 | 2.1 |
| 3 | 20 | 4.9 | 0.2 | 4.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 100.8 | 3.6 | 3.5 |
| 2 | 24 | 23.9 | 0.9 | 3.9 |
| 3 | 24 | 4.7 | 0.3 | 6.5 |
Background Information
Placenta growth factor (PlGF, also called PGF) is a member of the PDGF/VEGF family of secreted growth factors that share a conserved pattern of eight cysteines. PlGF is a homodimeric glycoprotein encoded by a single PlGF gene that gives rise to four splice isoforms in humans, PlGF-1 (PlGF131), PlGF-2 (PlGF152), PlGF-3 (PlGF203) and PlGF-4 (PlGF224). PlGF-1 and PlGF-2 represent the predominant isoforms. Unlike VEGF, PlGF homodimers bind VEGFR1, but not VEGFR2. PlGF exerts different non-redundant effects in stress conditions including wound healing, tissue ischemia and sepsis. PlGF-2 is expressed in human umbilical vein endothelial cells and in placenta.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AI854365, Pgf, PIGF, PIGF-2, placental growth factor, Plgf |