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Recombinant Mouse RAGE protein (His Tag)

ED50

/

Species

Mouse

Purity

>90 %, SDS-PAGE

GeneID

11596

Accession

Q62151-1

Cat No : Eg0873

Print datasheet

Synonyms

Ager,RAGE,MOK,RAGE 1,RAGE1

验证数据展示

  • Purity of Recombinant Mouse RAGE was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse RAGE was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Not tested
Source HEK293-derived Mouse RAGE protein Gly23-Ala340 (Accession# Q62151-1) with a His tag at the C-terminus.
Predicted Molecular Mass 34.6 kDa
SDS-PAGE 37-45 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

RAGE (receptor for advanced glycation endproducts), also called AGER, is a transmembrane receptor of the immunoglobulin super family. It mediates interactions of advanced glycosylation end products (AGE) which accumulate in vascular tissue in aging in diabetes. Acts as a mediator of vascular inflammation such as atherosclerosis and a complication of diabetes. AGE/RAGE signal regulates production or expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation. Interaction with S100B after myocardial infarction regulates myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. ABPP-initiated RAGE signaling, especially stimulation of P38 mitogen-activated protein kinase (MAPK), delivers ABPP as a complex with RAGE to the intraneuronal. RAGE has higher expression in lung tissues, in particular in alveolar type I cells, and is lost in idiopathic pulmonary fibrosis (IPF).

References:

1. Neeper M. et al. (1992) J Biol Chem. 267(21):14998-5004.
2. Hofmann MA. et al. (1999) Cell. 97(7):889-901.
3. Markus A. et al. (2008) Am J Respir Cell Mol Bio. 39(3):337-45.
4. Xue G. et al. (2008) Am J Physiol Heart Circ Physiol. 295(2):H491-8.
5. Takuma K. et al. (2009) Proc Natl Acad Sci U S A. 106(47):20021-6.
6. Fang F. et al. (2010) FASEB J. 24(4):1043-55.