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Recombinant Mouse Serpin E1/PAI-1 protein (His Tag)

ED50

/

Species

Mouse

Purity

>90 %, SDS-PAGE

GeneID

18787

Accession

P22777

Cat No : Eg1100

Print datasheet

Synonyms

PAI-1,Serpine1,PAI 1,PAI1,Plasminogen activator in

验证数据展示

  • Purity of Recombinant Mouse Serpin E1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Serpin E1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Not tested
Source HEK293-derived Mouse Serpin E1 protein Thr23-Pro402 (Accession# P22777) with a His tag at the C-terminus.
Predicted Molecular Mass 47.0 kDa
SDS-PAGE 42-48 kDa, reducing (R) condition
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Serpin E1, also known as plasminogen activator inhibitor 1 (PAI-1), is a protein that belongs to the serpin family of serine protease inhibitors. It is primarily produced by endothelial cells and plays a crucial role in the regulation of fibrinolysis by inhibiting the activity of plasminogen activators, such as tissue plasminogen activator (tPA) and urokinase (uPA). Serpin E1 is involved in a variety of physiological and pathological processes, including fibrinolysis, fibrosis, angiogenesis, wound healing, and the invasion and metastasis of tumor cells.

References:

1. Catarinella G. et al. (2022) Cell Death Dis. 13(8):737.
2. Meltzer ME. et al. (2010) Blood. 116(1):113-21.
3. Janciauskiene S. et al. (2024) Biomed Pharmacother. 175:116618.