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Recombinant Mouse TIGIT protein (His Tag)

ED50

16-65 ng/mL

Species

Mouse

Purity

>95 %, SDS-PAGE

GeneID

100043314

Accession

NP_001139797.1

Cat No : Eg0925

Print datasheet

Synonyms

T cell immunoreceptor with Ig and ITIM domains, TIGIT, VSIG9, VSTM3, WUCAM

验证数据展示

  • Purity of Recombinant Mouse TIGIT was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse TIGIT was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Mouse TIGIT (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD155 (hFc tag) with a linear range of 16-65 ng/mL.

    Immobilized Mouse TIGIT (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD155 (hFc tag) with a linear range of 16-65 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Mouse TIGIT (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD155 (hFc tag) with a linear range of 16-65 ng/mL.
Source HEK293-derived Mouse TIGIT protein Gly26-Thr143 (Accession# NP_001139797.1) with a His tag at the C-terminus.
Predicted Molecular Mass 13.7 kDa
SDS-PAGE 15-25 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells. It contains an immunoglobulin variable domain, a transmembrane domain, and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM.

References:

1.Xin Yu, et al. (2009) Nat Immunol. 10(1):48-57.
2.Noa Stanietsky, et al. (2009) Proc Natl Acad Sci U S A. 106(42):17858-63. 
3.Joe-Marc Chauvin, et al. (2020) J Immunother Cancer. 8(2):e000957.