Mouse VEGFR2/KDR ELISA Kit
Sensitivity
10.2 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10081 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse VEGFR2/Flk-1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse VEGFR2/Flk-1. The assay recognizes mouse VEGFR2/Flk-1. An antibody specific for mouse VEGFR2/Flk-1 has been pre-coated onto the microwells. The mouse VEGFR2/Flk-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse VEGFR2/Flk-1 is added to detect the captured mouse VEGFR2/Flk-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse VEGFR2/KDR ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Tissue homogenates |
| Assay type | Sandwich |
| Sensitivity | 10.2 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16542 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 93% | 83%-103% |
| Cell culture supernatants | 102% | 89%-114% |
| Tissue homogenates | 86% | 71%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1042.2 | 69.8 | 6.7 |
| 2 | 20 | 260.6 | 10.2 | 3.9 |
| 3 | 20 | 62.0 | 1.9 | 3.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 986.5 | 61.2 | 6.2 |
| 2 | 24 | 246.6 | 14.6 | 5.9 |
| 3 | 24 | 60.7 | 5.8 | 9.6 |
Background Information
VEGFR2, also named as KDR, FLK1 and CD309, is one of the two tyrosine kinase receptors involved in angiogenesis. VEGFR2 is mainly distributed in vascular endothelial cells, lymphatic endothelial cells, and embryonic precursor cells, and can bind to VEGF-A, VEGF-C, and VEGF-D. When activated by its ligand VEGF, VEGFR2 promotes neighbouring vessel formation to facilitate the delivery of growth factors, nutrients and oxygen for cancer proliferation, migration, metastasis and survival. VEGF and VEGFR2 mediated angiogenesis contributes to the aggressive natures and leads to high mortality rate in gastric cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 6130401C07, Flk 1, Flk1, Kdr, Krd 1, Ly73, VEGFR 2, VEGFR2 |