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验证数据展示

  • Single Cell Sequencing experiment G65053-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65053-1-5C on Resting PBMC

    G65053-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65053-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65053-1-5C on PBMC treated with 4hr LPS + TI

    G65053-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human CD107b/LAMP2 (H4B4)

CD107b / LAMP2 Monoclonal Antibody for Single Cell (Intra)
Cat No. G65053-1-5C

Print datasheet

CloneNo. H4B4

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

Single Cell (Intra)

Synonyms

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy LAMP2 Ready-To-Use IHC Kit. LAMP2 primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65053-1-5C targets CD107b / LAMP2 in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra) Application Description
Tested Reactivity Human
Immunogen Human adherent peripheral blood cells 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro® 5CFLX Anti-Human CD107b/LAMP2 (H4B4)
Synonyms CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker
Calculated Molecular Weight 45 kDa
GenBank Accession NumberBC002965
Gene Symbol LAMP2
Gene ID (NCBI) 3920
ENSEMBL Gene IDENSG00000005893
RRIDAB_3673905
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCCGTTCTCCATCTTGCCCATATAAGAAA
Barcode SequenceCCGTTCTCCATCTTG
Form Liquid
UNIPROT IDP13473
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol