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MultiPro™ 5CFLX Anti-Human CD11b (ICRF44)

CD11b Monoclonal Antibody for Single Cell (Intra), Single Cell

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

Single Cell (Intra), Single Cell

Conjugate

5CFLX

Barcode Sequence

CCAGTCAATAGCTAT

CloneNo.

ICRF44

Cat no : G65116-1-5C

Print datasheet

Synonyms

CD11B, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6

验证数据展示

  • Single Cell Sequencing experiment G65116-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65116-1-5C on Resting PBMC

    G65116-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65116-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65116-1-5C on PBMC treated with 4hr LPS + TI

    G65116-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65116-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65116-1-5C on Resting PBMC

    G65116-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD11B Ready-To-Use IHC Kit. CD11B primary antibody included.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65116-1-5C targets CD11b in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra), Single Cell Application Description
Tested Reactivity Human
Immunogen Rheumatoid synovial cells and human monocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Oligo Conjugate
Type Monoclonal
Full Name MultiPro™ 5CFLX Anti-Human CD11b (ICRF44)
Synonyms CD11B, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6
Calculated Molecular Weight 1152 aa, 127 kDa
GenBank Accession NumberBC096346
Gene Symbol CD11b
Gene ID (NCBI) 3684
ENSEMBL Gene IDENSG00000169896
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGCCAGTCAATAGCTATCCCATATAAGAAA
Barcode SequenceCCAGTCAATAGCTAT
Form Liquid
UNIPROT IDP11215
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C Stable for one year after shipment.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol