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验证数据展示

  • Single Cell Sequencing experiment G60253-1-5C on Resting PBMC

    Single Cell Sequencing experiment G60253-1-5C on Resting PBMC

    G60253-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G60253-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G60253-1-5C on PBMC treated with 4hr LPS + TI

    G60253-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G60253-1-5C on Resting PBMC

    Single Cell Sequencing experiment G60253-1-5C on Resting PBMC

    G60253-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

MultiPro® 5CFLX Anti-Human CD14 (2C1D9)

CD14 Monoclonal Antibody for Single Cell (Intra), Single Cell
Cat No. G60253-1-5C

产品说明书

CloneNo. 2C1D9

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

Single Cell (Intra), Single Cell

别名

CD14, CD14 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G60253-1-5C targets CD14 in Single Cell (Intra), Single Cell applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra), Single Cell Application Description
经测试反应性 Human
免疫原 CD14 fusion protein Ag10693 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD14 (2C1D9)
别名 CD14, CD14 molecule
计算分子量 375 aa, 40 kDa
GenBank蛋白编号BC010507
基因名称 CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
RRIDAB_3673900
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGATCGTCTGTTGCGCGCCCATATAAGAAA
条形码序列ATCGTCTGTTGCGCG
形式 Liquid
UNIPROT IDP08571
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol